Campylobacter jejuni is a major diarrhoeal pathogen. It possesses a number of virulence factors, but how they contribute to diarrhoea is not clear. There are no data on the prevalence of these virulence factors in C. jejuni isolated from diarrhoeal patients in Kuwait. In this study, we investigated some selected virulence factors. These included tissue culture adherence and invasion, cytolethal distending toxin (CDT) production and its gene (cdtB), and genes for invasion-associated marker (iam), invasion-associated secreted protein (ciaB) and invasion (cj0486), and resistance to ciprofloxacin (Resistance strains are reported to cause serious and prolonged infection). Twenty-nine selected diarrhoeal C. jejuni from patients at Mubarak Al-Kabir Hospital, Kuwait during 2007-2011 were studied. Adherence and invasion assays were done using Int-104 tissue culture cells. The input inoculum for the assays was 5 X 106-7 cfu per ml of the medium with tissue culture cells. Adherence was a 1 hour assay. For invasion, after 1 hour adhesion, the tissue culture cells were incubated with gentamicin for 3 hours (to kill extracellular bacteria), after which the tissue culture cells were lysed to enumerate intracellular bacteria. CDT production was assayed in HeLa cell monolayer. Specific PCR assays were done to detect the genes-cdtB, iam, ciaB and cj0486. Ciprofloxacin susceptibility was studied by E test. All 29 strains were both adherent and invasive for Int-104 cells. Twenty-seven strains were positive for cdtB gene, but only 13 expressed CDT production in HeLa cell assay. The numbers of isolates positive for cj0486, iam and ciaB genes were 10, 1, and 16 respectively . Fifteen strains were ciprofloxacin-resistant, but bore no relationship with adherence and invasion of Int-104 cells and CDT expression. There was a good correlation between adherence and invasion(r=0.66). Among the properties studied, the only correlation seen was between adherence and invasion. How the various virulence properties contribute to the disease process is not clear.