Angiostrongylus cantonensis is a metastrongyloid nematode found in lungs of various infected rat species. Infections with A. cantonensis in humans causes angiostrongyliasis which is characterised by an eosinophilic meningitis. Currently, A. cantonensis is the major cause of human eosinophilic meningitis worldwide. Intermediate hosts include a large variety of terrestrial molluscs, largely snails and slugs. Molluscs generally become infected following the consumption of rat faeces containing with the parasite. Humans are accidental hosts, becoming infected following the ingestion of infected molluscs, or contaminated food such as crustaceans or unwashed vegetables. The parasite is present in Australia, with several cases of angiostrongyliasis reported in children and young adults. The emergence of this zoonotic disease in recent years highlights the need for epidemiological studies aimed at determining the parasites’ prevalence in these areas. In response, this study aims to screen molluscs found in the Sydney metropolitan area by molecular screening. To date, 220 molluscs have been collected from various locations throughout the Sydney metropolitan area and these have undergone DNA extraction. Several positive samples have been identified using a sensitive real-time PCR (qPCR) which amplifies the Internal Transcribed Spacer-1 region (ITS1) of Angiostrongylus cantonensis. This survey will provide valuable information on the incidence of the parasite in the Sydney metropolitan area which will facilitate assessment of this parasites’ importance in this region.