In recent studies, tradition food materials, such as Antrodia cinnamomea will produce new efficiency compositions after fermentation, and then benefit our health for body or skin. In this research, we observed the functions of extraction with alcohol in Antrodia cinnamomea (AC901) containing stimulation cell proliferation, tyrosinase inhibitors. These results suggest that ethanol extraction of Antrodia cinnamomea reduces melanin synthesis by down-regulation of tyrosinase activity and serves as a new candidate in the design of new skin-care agents including in anti-oxidant, anti-aging, and whitening skin care products. In addition, AC901 nanofibrous mats were fabricated via electrospinning process. These fibers were blended with TiO2 nanoparticles (TiO2 NPs). The influence of TiO2 NPs on the nanofibrous matrices was investigated by scanning electron microscopy (SEM), transmission electron microscopy and thermogravimetric analysis. When the concentration of AC901 was up to 23% (w/v), the average diameter of the AC901/TiO2 fibers was 316 ± 49 nm by SEM images. These AC901/TiO2 nanofibrous mats exhibited higher equilibrium water content and water vapor transmission rate than hydrocolloid dressing. In addition, the cytocompatibility of AC901/TiO2 nanofibrous mats were analyzed by cell attachment, complete blood count and the spreading of L929 fibroblasts. We also demonstrated the functions of AC901/TiO2 mats and found it still inhibited tyrosinase activity and stimulation cell proliferation for 85% and 77%, respectively. These results suggested the novel nanocomposite (AC901/TiO2) mats may be used for skin care dressing.