Poster Presentation Australian Society for Microbiology Annual Scientific Meeting 2014

The effects of pilot-scale pulsed electric field processing on microbial and chemical milk quality during storage at 4 and 8°C. (#355)

Catherine M McAuley 1 , Jose Fernando Haro Maza 2 , Sieh Y Ng 1 , Tanoj K Singh 1 , Piotr Swiergon 1 , Roman Buckow 1
  1. CSIRO Animal, Food and Health Sciences, WERRIBEE, VIC, Australia
  2. Universidad de las Americas Puebla, Puebla, Mexico
In Australia, raw milk is required to undergo thermal processing so that the milk is safe to consume.  Recent interest in the consumption of raw milk and raw milk products has led to the consideration of alternative dairy processing technologies that will not compromise milk quality and safety.  One technology being investigated is pulsed electric field (PEF) processing, which can be used alone or in combination with thermal processing.  In the current study, PEF was investigated at two processing conditions and compared with two thermal treatments and a raw milk control.  PEF was conducted at ~30 kV/cm for a total of ~22 µs.  Outlet temperatures were either 63°C (P1) or 53°C (P2) for a total time of ~15 s before quickly cooling the milk to below 20°C.  Thermal controls were heated to either 72°C (T1, pasteurisation) or 63°C (T2, thermisation) for 15 s.  Milk bottles (1L) from each treatment were incubated for two weeks at 4 and 8°C, for a total of 20 bottles.  The milk was assessed for Total Plate Count, pH and lipid oxidation.  The entire experiment was replicated twice.  Raw milk was the quickest to reach spoilage (>106 cfu/mL) in 1-2 d at both temperatures.  At 4°C, T1 could last 10-14+ d, P1 could last 10-11 d, and T2 and P2 could last 6-7 d before spoilage levels were reached.  Storage at 8°C reduced the time until spoilage by approximately one half for the respective treatments.  A drop in pH occurred after a change in counts was observed.  Short chain acids dominated the volatile profile of the milk samples.  T1 and P1 followed similar trends and appeared most stable from volatile analysis, reflecting better quality indicated by lower total plate counts.  The PEF treatment (P1) has potential to be comparable to thermal pasteurisation (72°C for 15s).