The purpose of this study was to evaluate the use of the DiaSorin LIAISON chemiluminescent immunoassays for two microbiological assays previously performed by EIA. The LIAISON C.difficile GDH assay, a relatively new assay on the market (2013), was compared to the Techlab CDIFF CHEK-60 assay. The LIAISON Biotrin Parvovirus IgG and IgM assays were compared to reference laboratory results, which were obtained using the Siemens Novagnost Parvovirus B19 IgG and IgM ELISA. A total of 79 samples were used to evaluate the LIAISON C.difficile GDH assay, with an additional two samples used to test intra-run precision. There were no discordant results between the two GDH assays, giving 100% specificity and sensitivity in this evaluation of the LIAISON C.difficile GDH assay. The overall intra-run precision obtained in this study for the LIAISON GDH assay was CV 3.4%. In evaluating the LIAISON Biotrin Parvovirus assays, there was good agreement between the Biotrin Parvovirus IgG and IgM assays and the reference laboratory results. A total of 81 samples were retrospectively run on the LIAISON. A total of four discrepant results were seen – three samples had discrepant IgG results, and one had discrepant IgM result. The specificity and sensitivity of the IgG assay was 97.4% and 100% respectively. The specificity and sensitivity of the IgM assay was 100% and 88.9% respectively. The LIAISON provides an excellent alternative to EIA particularly for stool processing for GDH testing, as the procedure was simple and the assay automated, it removes user variability and reduces hands-on processing time. The Biotrin Parvovirus assays are well known and also recently converted to the LIAISON (2010). Its good performance in this evaluation gives confidence to change methods and gain associated cost savings from performing the test on-site.