Hydrophobic waxes crystalize into micro and nanostructures which form multifunctional assemblies on a great number of plants and insects. With their hydrophobic nature, they play a significant role in superhydrophobicity and self-cleaning on some natural surfaces which have inspired many biomimetic applications. Here, eicosane and docosane which are two of major components found on the surfaces of dragonfly wings were self-assembled on the surfaces of highly ordered pyrolytic graphite. The aliphatic hydrocarbons oriented epitaxial directions with the underlying graphite. Two types of bacterial cells, spherical-shaped Gram positive Staphylococcus aureus and rod-shaped Gram negative Pseudomonas aeruginosa, were then allowed to attach onto these surfaces. In both cases cells were only able to align to the microcrystals themselves and not to the underlying substratum in the spaces between. It was found that the microcrystal interfaces were able to entrap air pockets, which act to block cells from attaching to certain regions. This work describes a highly facile method for producing surfaces that control bacterial attachment via a hydroselective mechanism.