Pollution of hydrosphere with mercury and its attendant ecotoxicological consequences on public health are global concerns, which requires efficient mercury-removal strategies as panacea to the environmental problem. Exopolymeric substances (EPS) produced by highly resistant strains of Yarrowia spp. (Idd1 and Idd2) were isolated, characterized, and studied for their mercury removal potential. Mercury adsorptions were verified with thermodynamics, mass action equilibria, and kinetics of mercury uptake from simulated mercury-laden aquatic system onto the biopolymer. Excellent yield of EPS in medium supplemented with 3% glucose were observed with the Yarrowia strains (Idd1, 0.55 gdw l-1; Idd2, 0.51 gdw l-1) 7 d post-inoculation. Soluble EPS isolated from the yeasts consist mainly carbohydrates (387-409 mg gdw-1), protein (2.57-3.74 mg gdw-1), uronic acid (0.08-0.10 mg gdw-1), and nucleic acids (0.0018-0.0023 mg gdw-1). In contrast, contents of carbohydrates, proteins, and uronic acid were less in cell bound EPS, whereas, quantities of nucleic acids associated with cell bound EPS was 0.017-0.026 mg gdw-1. Mercury adsorptions by the EPS were well interpreted with Freundlich, Langmuir, and Brunauer-Emmer-Teller (BET) isotherms indicating 30-34 mg gdw-1 (approx.) achievable mercury uptake from aquatic system to form layers of mercury onto EPS colloids. The EPS apparently did not require much energy to adsorb mercuric ions from aquatic systems as depicted from their small activation energy (≤ 2.18 kJ mol-1). The adsorption models confirmed complexation of mercury by the EPS via heterogeneous multilayer adsorption of mercury. The EPS used chemisorption as rate-limiting step that controls the uptake of mercury from aquatic systems during micro-precipitation of mercuric ions as bioremoval strategy. The EPS produced by the two strains of Yarrowia are promising biotechnological tools in knowledge-based information required to design a bioreactor for treatment of mercury-laden industrial wastewater and bioremediation of aquatic environment polluted with mercuric ions.