Herpesviruses are ubiquitous, enveloped DNA viruses that have been identified worldwide in most vertebrate and in several invertebrate species. Although many herpesviruses of humans and domestic animals have been well characterised, many herpesviruses in wildlife species remain unidentified and their biological significance is unknown. We have recently undertaken a large study of different Australian wildlife species (over 600 animals), including macropods, koalas and wombats. This work has identified 10 novel herpesviruses (alphaherpesviruses and gammaherpesviruses) from a number different marsupial hosts, including koalas, common wombats, Tasmanian devils, bettongs, bandicoots, kangaroos and wallabies. Current work is focused on examining interactions between different pathogens, full genomic sequencing of selected viruses and investigating disease associations identified during epidemiological analyses.